Case Study 3: African clawed frog

(Xenopus laevis)

 Description

The African clawed frog is a highly resilient and adaptable freshwater amphibian native to sub-Saharan Africa. It is the most widely distributed amphibian in the world, found on five continents. Historically distributed globally for use in pregnancy tests and laboratories, it was listed as an Invasive Alien Species (IAS) of Union Concern in August 2024. It is a voracious predator with a very high reproductive rate and a cryptic habit.

Habitat

This species is highly mobile and can disperse through both water and over land. It is primarily aquatic, is a true habitat generalist, and often hides in remote agricultural ponds or thick vegetation, making it difficult to detect.

Impacts

  • Biodiversity: It is a fierce competitor that predates on native invertebrates, amphibians, and fish.
  • Disease: It serves as a subclinical carrier of the Chytrid fungus, a major global threat to native amphibians. While the frog itself is relatively tolerant to the pathogen, it is believed to transmit it to vulnerable local species.
  • Infrastructure: The species is known to invade houses and can clog water irrigation pipes.
  • Toxicity: They may be toxic to predators that attempt to eat them outside of their native range.

Prevention and Control Actions

The GuardIAS project is managing an expanding population moving from Northern France into Belgium. Because the species was only discovered in Belgium five years ago, there is still a high chance of successful eradication.

  • Advanced eDNA Detection: Researchers are developing a specialised multimarker eDNA barcoding protocol to improve detection sensitivity and eDNA quantification accuracy. This high-tech strategy allows teams to precisely identify breeding ponds even when frogs are hiding underwater.
  • Site Prioritization: Environmental DNA (eDNA) concentrations are used to select specific breeding ponds for management and to evaluate the success of control efforts afterward, judging from the difference in X. laevis eDNA concentrations after and before treatment.
  • Eradication Methods: Management includes trapping as well as quicklime application. This involves temporarily raising a pond’s pH level to remove the invasive frogs; the pH naturally resets to neutral over time, allowing the ecosystem to recover. Preliminary evidence shows quickliming can completely remove a X. laevis population. However, because landowners needed access to their ponds, associated amphibian fences had to be removed three months following treatment, enabling X. laevis recolonisation.
  • Landowner Cooperation: Since many affected ponds are on agricultural land, the project works closely with local farmers to explain the ecological importance of these actions and gain access to sites. Earlier research has shown that restricted access can severely undermine regional eradication programmes.

Useful links

Meet the case study team

Research Institute for Nature and Forest (INBO): Teun Everts and Rein Brys

Case study contacts:

  • Teun Everts, teun.everts@inbo.be
  • Rein Brys, rein.brys@inbo.be

Anastasija Zaiko

“This project is not just about managing invasive species today—it’s about developing a proactive approach that anticipates future ecological challenges, ensuring robust ecosystems and sustainable marine environments for generations to come”

Sergej Olenin

“For over 35 years, since I first began studying non-indigenous species, I have dreamed of tools that could reveal the ‘Big Picture’ of the transfer, spread, and impacts of invasive aquatic species. Thanks to advances in new technologies, the GuardIAS project now offers an outstanding opportunity to make that vision a reality.”

Anastasija Zaiko

Anastasija is a marine scientist with a broad expertise in aquatic ecology, ecology of biological invasions and the development and assessment of molecular tools for monitoring and surveillance. 

Anastasija is a co-founder and Lead Scientist of Sequench Ltd  (Nelson, New Zealand), specializing in molecular research and services as well as development of optimised sampling solutions for eDNA.

In 2023, she was elected a President of the newly established Southern eDNA Society (SeDNAs), which aims to promote science and industry collaboration to advance best practice eDNA methods and their wider adoption nature research and conservation.

Sergej Olenin

Marine biologist with over 40 years of experience in temperate seas and the Arctic. His research focuses on climate change, human impacts, and the spread of invasive alien species. He has pioneered international tools such as AquaNIS and other information system on IAS, IAS impact assessment and bioinvasion indicators.